Primer-Blast results - National Center for Biotechnology Information
Feb 4, 2025 · Primer-BLAST A tool for finding specific primers Making primers specific to your PCR template. more... Follow NCBI Connect with NLM
Basic Local Alignment Search Tool - BLAST
Jul 21, 2025 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and …
Primer designing tool - National Center for Biotechnology Information
Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type. Help Entrez query (optional) Help Primer specificity …
National Center for Biotechnology Information
Try "re-search for specific primers" feature on Primer-BLAST result page. When only non-specific primers are found, the result page offers the option to re-search for specific primers.
Primer-Blast results
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.
Primer-BLAST: A tool to design target-specific primers for polymerase ...
Users can design new primer pairs by entering the DNA template alone or they can design one primer by entering the template plus the other pre-existing primer. Primer-BLAST can check the specificity …
Molecular basis for the initiation of DNA primer synthesis
The structural and mechanistic studies presented here establish how Prim-Pol proteins instigate primer synthesis, revealing the requisite molecular determinants for primer synthesis within the catalytic …
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Primer designing tool - National Center for Biotechnology Information
The option "Primer must span an exon-exon junction" will direct the program to return at least one primer (within a given primer pair) that spans an exon-exon junction.
Primer-design for multiplexed genotyping - PubMed
Mar 15, 2003 · Such high-throughput scoring of SNPs requires a high level of primer multiplexing in order to analyze multiple loci in one assay, thus enabling inexpensive and fast polymorphism …