Comet assay - Wikipedia
The single cell gel electrophoresis assay (SCGE, also known as comet assay) is an uncomplicated and sensitive technique for the detection of DNA damage at the level of the individual eukaryotic cell. It was first developed by Östling & Johansson in 1984 and later modified by Singh et al. in 1988. [ 1 ]
The comet assay: a method to measure DNA damage in individual …
Jun 27, 2006 · We present a procedure for the comet assay, a gel electrophoresis–based method that can be used to measure DNA damage in individual eukaryotic cells. It is versatile, relatively simple to...
Comet assay: a versatile but complex tool in genotoxicity testing
The comet assay is a versatile method for measuring DNA strand breaks in individual cells. It can also be applied to cells isolated from treated animals. In this review, we highlight advantages and limitations of this in vivo comet assay in a ...
The comet assay for DNA damage and repair: principles ... - PubMed
The comet assay (single-cell gel electrophoresis) is a simple method for measuring deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form nucleoids containing supercoiled loops of DNA linked to the …
We describe here recent technical develop-ments, theoretical aspects, limitations as well as advantages of the assay, and modifications to measure cellular antioxi-dant status and different types of DNA repair. We briefly describe the applications of this method in genotoxicity testing, human biomonitoring, and ecogenotoxicology.
Measuring DNA Damage Using the Alkaline Comet Assay in …
The comet assay is a widely used method that allows the measurement of both DNA damage and its repair in cells. For this, cells are treated with DNA-damaging agents and embedded into a thin layer of agarose on top of a microscope slide.
The comet assay: past, present, and future - PMC - PubMed …
The alkaline comet assay (single cell gel electrophoresis) is the most widely used method for measuring DNA damage in eukaryotic cells (Neri et al., 2015). It detects strand breaks (SBs) and alkali-labile sites at frequencies from a few hundred to several thousand breaks per cell—a biologically useful range, extending from low endogenous ...
The comet assay: past, present, and future - Frontiers
Aug 12, 2015 · Since the first report by Ostling and Johanson (1984) the comet assay has been widely used in genotoxicity testing of chemicals, in both in vitro and in vivo models. An advantage with the latter is that cells from various tissues can be studied, in a …
Measuring DNA modifications with the comet assay: a …
Jan 27, 2023 · The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human.
The comet assay, also known as single-cell gel electrophoresis (SCGE), has become an attractive assay in the assessment of DNA damage. Its simplicity, sensitivity, speed and economy make it a prime choice in genotoxicity testing, ecogenotoxicity, human epidemiology studies as well as mechanistic studies of DNA damage and repair.
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