Traditional methods to determine enzyme kinetics are: continuous assays, where the rate is determined by spectroscopic measurement of product generation (or substrate depletion), discontinuous ...

Furthermore, the stable depletion of IRS-2 strongly decreased ... IGF-1R, IGF-1 receptor; IRS, insulin receptor substrate; MEF, mouse embryo fibroblast; PH, pleckstrin homology; PI3-K ...