HER2+ breast cancer usually undergo preoperative therapy with multi-agent chemotherapy in combination with anti-HER2 ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and ...

announces its recent publication in Nature Communications describing a massively multiplexed PCR primer design algorithm, named Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE).

Genome walking is a method used to retrieve unknown flanking DNA. Here, we reported wristwatch (WW) PCR, an efficient genome walking technique mediated by WW primers (WWPs). WWPs feature 5′- and ...

To do this, researchers make serial dilutions of their PCR reagents, changing one variable at a time to find the optimal concentrations. Additionally, different annealing temperatures beyond the one ...

Both still use several primers. Diagram showing the multiplex PCR approach, whereby all of the primers are added to a single tube. Image credit: S. Rosel, 2021 Annealing temperatures for each of the ...