The recommended final concentration is 1-10 percent. Formamide weakens base pairing and increases primer annealing specificity, thus enhancing PCR amplification for templates with high GC content.

PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...

PCR is very precise and can be used to amplify ... The mixture is then cooled so that the primers anneal, or bind, to the DNA template. At this point, the DNA polymerase begins to synthesize ...

since the annealing temperature of the fluorescent dye-labeled M13(-21) primer is only 53°C, the last eight PCR cycles should be run with an annealing temperature of 53°C (the same result can be ...

You could just put everything in the tube, close it, and cycle the temperature, and PCR would occur.” Additionally, raising the annealing and elongation temperatures increased primer binding ...

Scorpions Primers utilize a uni-molecular mechanism that acts faster in solution for instantaneous fluorescence in real-time PCR. Like Molecular Beacons, these primers do not require enzymatic ...