detection techniques and reproducibility. Western blotting is a staple technique in many laboratories but can be time consuming and inconsistent. Technology Networks spoke with Harsha Rao to learn ...

Western blotting ... to enable their detection. The challenge is this can likely result in the HKP being outside its linear dynamic range. This discrepancy is often not accounted for in western blot ...

To further understand the cell signaling involved in the Ang2-mediated inhibition of metastasis, immunofluorescence staining was ... Reliably quantified Western blotting was required to support ...

If scientists understand how Western ... blot to prevent it from drying out. Most of the time, Gomes says the problem is in the amount of antibody. If the concentration is too dilute, no detection ...

The No-Stain reagent does not require any special gels or reagents and is compatible with existing gel staining and western blotting workflows. We present data and analysis demonstrating the ease of ...

This article describes the histone western blot protocol, which is often used ... ECF, ECL, or infrared detection is carried out as recommended by the manufacturer, for example, ECL reagents ...

On the other hand, Western blot requires that samples are first separated by electrophoresis and then immobilized in a blotting membrane. The method of sample staining is also slightly different ...