The company’s Syndigo1 facility in Connersville, Indiana, has an estimated production capacity of more than 100 million ...

This useful study explores the role of RAP2A in asymmetric cell division (ACD) regulation in glioblastoma stem cells (GSCs), drawing parallels to Drosophila ACD mechanisms and proposing that an ...

INTEGRA Biosciences offers a variety of solutions to streamline crucial PCR purification workflows with magnetic beads and ...

By applying the algorithm to a nine-wavelength-band measurement of four-capillary electrophoretic separation of STR-PCR (short tandem repeat-polymerase chain reaction) products labeled with six dyes, ...

The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

PCR involves three core components: polymerase, primer, and template. Primers anneal to templates and create initiation sites for the polymerase. The polymerase then recruits free deoxynucleoside ...

Isolation of total ribonucleic acid and real-time RT-PCR Total ribonucleic acid (RNA ... 95°C for 10 min followed by 40 cycles of denaturation at 95°C for 15 s and primer annealing at 62°C for 1 min ...

Sequences of primers and probes for the real-time PCR assays designed to amplify specific genomic ... followed by 45 cycles of denaturation at 95°C for 1 s and annealing/extension at 60°C for 20 s.

PCR amplifications consisted of 30 cycles at 94°C for 1 min, annealing temperature specific for each primer for 1 min (Table 1) and 72°C for 2 min in a Thermal Cycler (Gene Amp PCR System 9700/Perkin ...