A method for genetically barcoding Drosophila is developed and used to tag defined cell populations in vivo for single-cell transcriptomics experiments and to enable multiplexed behavioral analysis.
PCR was developed in 1983 ... DNA or cDNA as a template during repeated cycles of heat denaturation, primer annealing and primer extension. With each cycle the amount of template DNA is doubled ...
This study provides a valuable new resource to investigate the molecular basis of the particular features characterizing the pipefish embryo. The authors found both unique and shared gene expression ...
A final annealing step of 72°C for 5 min was performed. The ITS region of the fungal 18S r RNA gene was targeted using ITS1 universal primers (F: 5′- GGAAGTAAAAGTCGTAACAAGG-3′, R: 5′- ...
The fungal yield was evaluated using a broad-range real-time PCR assay as described previously (Nemcova et al., 2015) using the Rotor-Gene 6000 (Corbett Research, Austria). Briefly, amplification of ...
Subsequently, the reverse transcriptase reaction mixture was used for PCR amplification with forward and reverse oligonucleotide primers, using a PCR kit with ... heat denaturation (94°C for 1.5 ...
Download this application note to learn how the microbial detection capabilities of real-time PCR-based bacterial and fungal kits compare to those of culture-based sterility testing. You can withdraw ...
Despite growing interest in using PCR materials in packaging to reduce virgin plastic use, producers and recyclers report ...
Oprah is a fan of the brand, too. Perfect for mature skin, Laura Geller’s hydrating primer comes in a rich cream-to-gel formula that’s super moisturizing yet lightweight. It’s ideal for ...
Finding the right primer for dry skin is a challenge for us all – yes, including us beauty journos (hey 👋). If you're working with a flakey complexion, you'll know how annoying it is when ...
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