The primer melting temperature (Tm) should be the same for all primers used in the multiplex reaction. Plan to amplify a 75–200 bp product. Short PCR products are typically amplified with higher ...

Amcor's investment will enhance its capabilities for producing post-consumer recycled (PCR) packaging, offering customers ...

Primers need to be aligned to annotated assemblies of the genome of interest (template DNA of LAM-PCR) to avoid unspecific hybridization of the primers.

If so, the PCR primer would preferentially anneal to and amplify the chromosome with the perfectly matched allele.

The rapid identification of respiratory virus outbreaks is needed to enable rational and effective public health interventions. We developed new quantitative approaches for simultaneous ...

The authors provide solid evidence of its utility by benchmarking multiple demultiplexing methods and proposing a new variant consistency metric. While the tool is valuable for guiding method ...