High-yield requirements might call for cloning of the target gene into an Escherichia coli expression vector, whereas subsequent activity studies might benefit from insect or mammalian cell expression ...

However, this has proved challenging to achieve because the Cre gene in the vector is spontaneously expressed in the E. coli bacteria used to synthesize such a vector, causing recombination to ...

coli. Berg wanted to develop tools to understand gene expression in human cells and ... improved cloning vector to show that frog DNA could also be replicated and transcribed in E. coli. While Paul ...

The Overnight Express Autoinduction Systems enable regulated protein expression in Escherichia coli without the need to monitor the culture or add inducer during cell growth and are marketed ...

A team recently detailed an auto-expression medium to maximize protein production that uses galactose rather than lactose to induce auto-expression.

This vector allows the creation of Cre-loxP ... thereby preventing its expression in E. coli. They also demonstrated that this technology can be applied to newts and mice. While continuing their ...

In this study, the researchers succeeded in synthesizing a Cre-loxP integrated vector by placing a short ... thereby preventing its expression in E. coli. They also demonstrated that this ...