One-step creation of Cre-loxP organisms: Precise control and modification of gene expression
Researchers at the University of Tsukuba have developed a vector that integrates the Cre enzyme gene with the specific DNA ...
The Scientist1y
Have It Three Ways
High-yield requirements might call for cloning of the target gene into an Escherichia coli expression vector, whereas subsequent activity studies might benefit from insect or mammalian cell expression ...
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WuXi Biologics introduces E coli expression system
WuXi Biologics has launched the E coli expression system, EffiX, designed to enhance the production of recombinant proteins and plasmid DNA (pDNA). The worldwide market for microbial-derived ...
The Scientist1y
Choosing the Best Reporter Assay
Reporter genes are generally joined to a regulatory DNA sequence in an expression vector that is usually propagated in Escherichia coli before transfection into the cell type of interest. 1 A control ...
Nature1y
The Overnight Express Autoinduction System: High-density cell growth and protein expression while you sleep
The Overnight Express Autoinduction Systems enable regulated protein expression in Escherichia coli without the need to monitor the culture or add inducer during cell growth and are marketed ...
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One-step creation of Cre-loxP organisms: precise control and modification of gene expression
In this study, the researchers succeeded in synthesizing a Cre-loxP integrated vector by placing a short ... thereby preventing its expression in E. coli. They also demonstrated that this ...